Doxorubicin Efflux Pump in Archaebacteria Similar to P-glycoprotein
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چکیده
منابع مشابه
Doxorubicin-efflux pump in Haloferax vokanii is energized by ATP
Haloferax uolcanii acquires resistance to doxorubicin, and this acquisition involves an active extrusion of lipophilic drugs. We have investigated what this drug-efflux transporter utilizes as an energy source to expel drugs from the cells. The efflux activity was estimated from the direct measurement of drug efflux rate and from the steady-state level of drug accumulation. Cells treated with N...
متن کاملExistence of a p-glycoprotein drug efflux pump in cultured rabbit conjunctival epithelial cells.
PURPOSE To determine whether a p-glycoprotein (P-gp) drug efflux pump exists in cultured rabbit conjunctival epithelial cells (RCEs) to restrict the absorption of cyclosporin A (CSA) and other lipophilic drugs such as verapamil and dexamethasone. METHODS The anti-P-gp monoclonal antibody (mAb) C219 was used in western blot analysis to reveal the presence of P-gp in freshly isolated and cultur...
متن کاملIn Situ Biochemical Demonstration That P-Glycoprotein Is a Drug Efflux Pump with Broad Specificity
While P-glycoprotein (Pgp) is the most studied protein involved in resistance to anti-cancer drugs, its mechanism of action is still under debate. Studies of Pgp have used cell lines selected with chemotherapeutics which may have developed many mechanisms of resistance. To eliminate the confounding effects of drug selection on understanding the action of Pgp, we studied cells transiently transf...
متن کاملDrug transport to the brain: key roles for the efflux pump P-glycoprotein in the blood-brain barrier.
1. The blood-brain barrier (BBB) contributes to brain homeostastis and fulfills a protective function by controlling the access of solutes and toxic substances to the central nervous system (CNS). The efflux transporter P-glycoprotein (P-gp) is a key element of the molecular machinery that confers special permeability properties to the BBB. 2. P-gp, which was initially recognized for its abilit...
متن کاملEffects of Astragalus polysaccharides on P-glycoprotein efflux pump function and protein expression in H22 hepatoma cells in vitro
BACKGROUND Astragalus polysaccharides (APS) are active constituents of Astragalus membranaceus. They have been widely studied, especially with respect to their immunopotentiating properties, their ability to counteract the side effects of chemotherapeutic drugs, and their anticancer properties. However, the mechanism by which APS inhibit cancer and the issue of whether that mechanism involves t...
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ژورنال
عنوان ژورنال: Drug Metabolism and Pharmacokinetics
سال: 1993
ISSN: 0916-1139
DOI: 10.2133/dmpk.8.supplement_731